Human immunodeficiency virus (HIV) infection is a major global health concern and social problem. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Ĭompeting interests: The authors have declared that no competing interests exist. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.ĭata Availability: All relevant data are within the manuscript.įunding: This study was supported by the Hubei Province Health and Family Planning Scientific Research Project (No: WJ2019M014 to M-QL) received by Dr. Received: FebruAccepted: AugPublished: September 11, 2020Ĭopyright: © 2020 Liu et al. PLoS ONE 15(9):Įditor: Qigui Yu, Indiana University School of Medicine, UNITED STATES (2020) Anti-HIV-1 antibodies based confirmatory results in Wuhan, China, 2012-2018. Our results indicated the detection rate had decreased for HIV-1 infection, but increased for suspected recent and acute HIV-1 infection during 2012–2018, reflecting the efforts of intervention among high risk population.Ĭitation: Liu P, Tang L, Kong W-H, Zhu Z-R, Xiao P, Wang X, et al. The suspected acute HIV-1 infection was also observed to be increased in recent years, with an overall rate of 1.00%. The most commonly observed antibody profile was gp160+gp120+p66+(p55+)p51+gp41+p31+p24+p17+ (4131, 49.43%) for WB-MP and gp160+gp120+gp41+p31+p24+p17+ (382, 58.59%) for RIBA-WANTAI, and the absence of reactivity to three possible serologic markers for recent HIV-1 infection, p31, p66, and p51, increased significantly from 2012 to 2018, with the overall rate of 17.03%, 9.40%, and 15.15%, respectively. Although the number of HIV-1 infected subjects increased significantly from 2012 (n = 911) to 2018 (n = 1578), the positive rate of HIV-1 antibodies decreased markedly from 70.08% in 2012 to 58.79% in 2018 ( p<0.001). The indeterminate rate of WB was significant higher than that of RIBA ( p<0.001).
RIBA had identified 652 (81.09%) positive and 13 (1.62%) indeterminate cases from Jto January 7, 2015, while WB had identified 8358 (81.43%) positive and 643 (6.26%) indeterminate cases in the other times during 2012–2018. A total of 14432 HIV-suspected serum or plasma samples collected from local hospitals and other HIV screening laboratories were further screened by two 4 th generation enzyme-linked immunosorbent assay (ELISA) kits in our laboratory, of which 11068 specimens (76.69%) had at least one positive ELISA result and thereby were finally confirmed with WB or RIBA. In this study, we retrospectively analyzed the HIV-1 confirmatory results tested by western blot (WB) or recombination immunoblot assay (RIBA) in Wuhan, 2012–2018, to access the profiles of HIV-1 specific antibodies. The number, intensity and order of emergence of HIV-1 specific antibodies in serum or plasma were associated with the stage of HIV-1 infection.
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